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KMID : 0380020180330040293
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Korean Journal of Biotechnology and Bioengineering
2018 Volume.33 No. 4 p.293 ~ p.298
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Apoptosis Effects of Extract from Allium hookeri by AMPK/Akt/ GSK-3β Signal Pathway in AGS Gastric Cancer Cells
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Park Ye-Seul
Nam Gun-He
Jo Kyung-Jo
Kim Sang-Yung
Wee Ji-Hyang
Kim Young-Min
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Abstract
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Allium hookeri is distributed across Asia and is known to possess antioxidant and anti-inflammatory properties. AMPK is activated by ATP consumption or nutrient deficiency. It plays an important role as an energy sensor and apoptosis regulator. Moreover, Akt plays an important role in cell growth and division. It also regulates apoptosis-mediated proteins such as p53 and GSK-3β. GSK-3β, which is involved in growth metabolism, and various other transcription factors determine the survival or death of an organism. We therefore evaluated whether A. hookeri extract could inhibit cell proliferation and induce apoptosis through the AMPK/Akt/GSK-3β signaling pathway by performing MTT assay, western blotting, and Annexin V & Dead cell staining. MTT assay results revealed that AHE could effectively inhibit the AGS gastric cancer cells in dose-dependent manner (90, 135, 180, 225μg/ ml) but not that of fibroblast cells (90, 135, 180, 225μg/ml). In other words, its action was unique to stomach cancer cells. Annexin V & Dead cell staining confirmed increase in the rate of apoptosis in a dose-dependent manner (90, 135, 180μg/ml). Furthermore, we identified that AHE decreased the expression of p-Akt, GSK-3β, b-catenin, pro caspase 3, and Bcl-2 anti-apoptotic proteins, while it increased those of p-AMPK, p53, cleaved PARP, cleaved caspase 3, and Bax pro-apoptotic proteins. Our cumulative results suggest that AHE decreases AGS cell proliferation and induces apoptosis by inhibiting Akt and AMPK expression.
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KEYWORD
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Allium Hookeri, AGS cancer cell, apoptosis, AMPK/ AKT/GSK-3β pathway
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